LIFR Knockout cell line(NCI-H1299)

ENCN
location： Home > Products > KO Cell Lines > LIFR Knockout cell line(NCI-H1299)

LIFR Knockout cell line(NCI-H1299) Order

LIFR Knockout cell line(NCI-H1299)
Catalog#
YKO-H318
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
LIFR Knockout cell line(NCI-H1299)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
LIFR Knockout cell line (NCI-H1299)
Cell Morphology
Epithelial-like, adherent
Passage ratio
1:2~1:3
Species
Human
Gene Name
LIFR
Gene ID
3977
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
88%RPMI-1640+10% FBS+1%Glutamax+1%Sodium Pyruvate
Accession
CVCL_E0ZL(Cellosaurus)
ExPASy Cellosaurus Database
Is a valuable online resource that compiles detailed information on over 100,000 cell lines from reputable cell banks such as ATCC, ECACC, and DSMZ. The database provides information including descriptions, origins, and characteristics of the cell lines, with broad reference value and scientific reliability. Additionally, the database provides information on cell line authentication, STR profiles, and potential contamination risks, helping researchers ensure the trustworthiness of the cell lines used in their experiments.
Target region
Knockout region: Exon 6 (based on transcript LIFR-202)
Validation: PCR+Sanger Sequencing
LIFR Knockout cell line(NCI-H1299) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:NCI-H1299		 STR Info (Cell bank)
Cell Line:NCI-H1299-Luc2-tdT-2
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11111111
D13S317 12121212
D7S820 10101010
D16S539 12131213
VWA 16181618
TH01 69.369.3
AMEL XXXX
TPOX 8888
CSF1PO 12121212
D12S391 2122
FGA 2020
D2S1338 2324
D21S11 32.232.2
D18S51 1616
D8S1179 1013
D3S1358 1717
D6S1043 1111
PENTAE 1111
D19S433 1414
PENTAD 1313
D1S1656 1215
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the LIFR gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the NCI-H1299 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, LIFR gene knockout LIFR Knockout cell line (NCI-H1299) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
LIFR Knockout cell line(NCI-H1299)
LIFR Knockout cell line(NCI-H1299)
Catalog#
YKO-H318
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • LIFR Knockout cell line (HeLa)

    LIFR Knockout cell line (HeLa)
      inquiry   More details

    Organism: Human
    Gene Name: LIFR
    Gene ID: 3977
    Size: 1*10^6
    Catalog#: YKO-HS3639
    YKO-HS3639(HeLa)
  • LIFR Knockout cell line (HCT 116)

    LIFR Knockout cell line (HCT 116)
      inquiry   More details

    Organism: Human
    Gene Name: LIFR
    Gene ID: 3977
    Size: 1*10^6
    Catalog#: YKO-HS2516
    YKO-HS2516(HCT 116)
  • LIFR Knockout cell line (HEK293)

    LIFR Knockout cell line (HEK293)
      inquiry   More details

    Organism: Human
    Gene Name: LIFR
    Gene ID: 3977
    Size: 1*10^6
    Catalog#: YKO-HS1025
    YKO-HS1025(HEK293)

Related Popular Products

Wild-type Cell Lines

  • NCI-H1299 cell line

    Human Non-small Cell Lung Cancer Cell Line(NCI-H1299)

    Price (USD): -
    Catalog#: YC-D010
    Size: 1*10^6
    Instruction: Human Non-small Cell Lung Cancer Cell Line(NCI-H1299)
      inquiry   More details

Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • LIFR Knockout cell line (HeLa)

    LIFR Knockout cell line (HeLa)

    Organism: Human
    Gene Name: LIFR
    Gene ID: 3977
    Size: 1*10^6
    Catalog#: YKO-HS3639
      inquiry   More details
  • LIFR Knockout cell line (HCT 116)

    LIFR Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: LIFR
    Gene ID: 3977
    Size: 1*10^6
    Catalog#: YKO-HS2516
      inquiry   More details
  • LIFR Knockout cell line (HEK293)

    LIFR Knockout cell line (HEK293)

    Organism: Human
    Gene Name: LIFR
    Gene ID: 3977
    Size: 1*10^6
    Catalog#: YKO-HS1025
      inquiry   More details
×

Subscribe Us

By subscription, you consent to allow Ubigene Biosciences to store and process the information provided above to deliver the latest news, research spotlight, and promotions. You can unsubscribe from these communications at anytime.
WhatsApp: +86 153 6067 3248
TEL: +86 153 6067 3248 (Int'l)
US Toll free: 855 777 3210
EU Toll free: 800 3272 9252
Korea Toll free: 001 800 3272 9252

Gene Editing Services

EZ-editor™ Products

Copyright © 2024 Ubigene. All rights reserved.
×

Search documentation

Literature:

Name: *

Company: *

Telephone: *

Email:

Notes: After submitting the order, we will contact you as soon as possible.

request now